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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
06/01/2009 |
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Data da última atualização: |
17/04/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRITO, L. G.; OLIVEIRA, M. C. de S.; ROCHA, R. B.; SILVA NETTO, F. G.; CAVALCANTE, F. A. |
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Afiliação: |
Luciana Gatto Brito, Embrapa Rondônia; Márcia Cristina De Sena Oliveira, Embrapa Pecuária Sudeste; Rodrigo Barros Rocha, Embrapa Rondônia; Francelino Goulart da Silva Netto, Embrapa Rondônia; Francisco Aloísio Cavalcante, Embrapa Acre. |
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Título: |
Estudo da divergência genética em linhagens brasileiras de anaplasma marginale através da utilização do gene da proteína principal de superfície MSP1a. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008. |
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Idioma: |
Português |
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Notas: |
Meta 2008. |
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Conteúdo: |
Bovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 µl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of msp1a gene, five rickettsia isolated types were identified in the A. marginale populations from São Paulo, Rondônia and Acre states. These results indicates that the strains of Acre state were less similar with São Paulo sample and not have divergence within strains obtained of samples provided MenosBovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 µl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of ... Mostrar Tudo |
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Palavras-Chave: |
Anaplasma marginele; Estudo genético advergência. |
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Thesagro: |
Gene; Proteína. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02650naa a2200229 a 4500
001 1709264
005 2009-04-17
008 2008 bl --- 0-- u #d
100 1 $aBRITO, L. G.
245 $aEstudo da divergência genética em linhagens brasileiras de anaplasma marginale através da utilização do gene da proteína principal de superfície MSP1a.
260 $c2008
500 $aMeta 2008.
520 $aBovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 µl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of msp1a gene, five rickettsia isolated types were identified in the A. marginale populations from São Paulo, Rondônia and Acre states. These results indicates that the strains of Acre state were less similar with São Paulo sample and not have divergence within strains obtained of samples provided
650 $aGene
650 $aProteína
653 $aAnaplasma marginele
653 $aEstudo genético advergência
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aROCHA, R. B.
700 1 $aSILVA NETTO, F. G.
700 1 $aCAVALCANTE, F. A.
773 $tIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
03/01/2007 |
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Data da última atualização: |
31/03/2015 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
DALLA COSTA, O. A.; COSTA, M. J. R. P. da; FAUCITANO, L.; KICH, J. D.; ARMILIATO, N. M.; TRIQUES, N. J.; COLDEBELLA, A.; LUDKE, J. V.; PELOSO, J. V.; HOLDEFER, C.; BALDI, P. C.; ROZA, D. D. |
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Afiliação: |
OSMAR ANTONIO DALLA COSTA, CNPSA; MATEUS JOSE RODRIGUES PARANHOS DA COSTA, FCAV-UNESP; LUIGI FAUCITANO, Agriculture and Agri-Food Canadá. Quebec, Canadá.; JALUSA DEON KICH, CNPSA; NEILOR MANOEL ARMILIATO, CNPSA; NELISE TRIQUES, UNC/CNPSA; ARLEI COLDEBELLA, CNPSA; JORGE VITOR LUDKE, CNPSA; JOSE VICENTE PELOSO, SADIA SA. Concórdia, SC; CARMO HOLDEFER, CNPSA; PAULO CESAR BALDI, CNPSA; DARLAN DALLA ROZA, TRIEL.HT Industria de Equipamentos Rodoviários LTDA. Erechim, RS. |
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Título: |
Efeito do tempo de jejum dos suínos na granja sobre o bem-estar, medido pelo cortisol na saliva e pela frequência cardíaca, durante o manejo pré-abate. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Concórdia: Embrapa Suínos e Aves, 2006. |
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Páginas: |
3 p. |
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Série: |
(Embrapa Suínos e Aves. Comunicado Técnico, 439). |
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Idioma: |
Português |
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Palavras-Chave: |
Manejo pré abate. |
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Thesagro: |
Bem-Estar; Suíno. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPSA/15861/1/publicacao_p9p49c0o.pdf
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Marc: |
LEADER 00894nam a2200289 a 4500
001 1443744
005 2015-03-31
008 2006 bl uuuu u0uu1 u #d
100 1 $aDALLA COSTA, O. A.
245 $aEfeito do tempo de jejum dos suínos na granja sobre o bem-estar, medido pelo cortisol na saliva e pela frequência cardíaca, durante o manejo pré-abate.
260 $aConcórdia: Embrapa Suínos e Aves$c2006
300 $a3 p.
490 $a(Embrapa Suínos e Aves. Comunicado Técnico, 439).
650 $aBem-Estar
650 $aSuíno
653 $aManejo pré abate
700 1 $aCOSTA, M. J. R. P. da
700 1 $aFAUCITANO, L.
700 1 $aKICH, J. D.
700 1 $aARMILIATO, N. M.
700 1 $aTRIQUES, N. J.
700 1 $aCOLDEBELLA, A.
700 1 $aLUDKE, J. V.
700 1 $aPELOSO, J. V.
700 1 $aHOLDEFER, C.
700 1 $aBALDI, P. C.
700 1 $aROZA, D. D.
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Embrapa Suínos e Aves (CNPSA) |
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